Here we recommend alignment goals for good performance. If you are interested in acquiring this objective then please contact Jon Daniels at Applied Scientific Imaging (ASI) directly: (or Drawings Mechanically limited field of view diameter (1 direction only) 40x with 200 mm tube lens)ĭiffraction limited field of view diameter The optical specifications are as follows:Įffective focal length (e.g. A CAD rendering b) of the design and a photo c) of the final working lens. A drawing a) shows the critical opto-mechanically coupled design considerations for the bespoke objective: to collect a tilted air space image just ~100 μm from a flat surface, with a range of tilt angles from 0-45 degrees. Figure A1: Bespoke glass-tipped objective. The optical performance and geometry of this lens allow it to extract a high resolution image as close as ~100 μm to a flat surface, with negligible loss in resolution and high transmission, over a range of tilt angles from 0-45 degrees. By choosing all stock parts throughout the design of the rest of the microscope many of the opto-mechanical difficulties are compressed into the design of this final objective (Figure A1). The critical enabling component of the high NA single-objective light-sheet microscope is the bespoke glass-tipped objective. Preferred with typical live cell refractive index: 1.35 to 1.4 If you are interested in item 11 the glass-tipped objective (O3) then please see the following section on the AMS-AGY objective v1.0. Item 10, the small glass window (O2*), can be exchanged for a standard coverslip for limited budgets. All of the optics are 'stock' parts except for items 10 (O2*) and 11 (O3) which are currently 'bespoke'. For clarity, the elements are listed in order in which they are physically arranged (from sample to camera). This table can be used as a shopping list for the original design of the emission path. Here we label the single-objective light-sheet schematic and highlight the key components and parameters that are useful for disucsions of design, build and alignment.
(to update) SOLS parameters: a reference diagram for key parameters.
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For the full version of this article, please visit one of the following links: High NA single-objective light-sheet Note that this is a limited PDF or print version animated and interactive figures are disabled.
This project is maintained by amsikking in the York lab, and was funded by Calico Life Sciences LLC (in progress) Appendix
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